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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IP |
|---|---|
| Reactivity | Bovine, Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-97 |
| Immunogen | Raji human Burkitt's lymphoma cell line |
| Target | CD20 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B1, S7, MS4A, Bp35, CVID5, LEU-16
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Flow cytometry analysis (surface staining) of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD20 (MEM-97) antibody.
Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-CD20 rabbit polyclonal antibody. Lane 1: original lysate of RAJI cells Lane 2: immunoprecipitate by Isotypic mouse IgG1 control (PPV-06; cat. no. orb43531) Lane 3: original lysate of RAJI cells Lane 4: immunoprecipitate by anti-CD20 (MEM-97).
Separation of human CD20 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD20 (MEM-97) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD20 (MEM-97) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.
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Protocol Information
FC
Flow Cytometry
IP
Immunoprecipitation
CD20 Antibody (orb44418)
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