CD222 Antibody (Biotin)

SKU: orb402816

Description

Mouse monoclonal antibody against CD222 conjugated to biotin

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	FC, IP, WB
Reactivity	Human, Primate
Application Notes	Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2-6 μg/ml.Western blotting: Recommended dilution: 1 µg/ml; non-reducing conditions.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	MEM-238
Immunogen	Recombinant Vaccinia virus encoding CD222.
Target	CD222
Purification	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Store at 2-8°C. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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IGF2R, MPR1, CIMPR, MPR300, M6P-R

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Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC.

Anti-Hu CD222 Biotin (clone MEM-238) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Jurkat, HeLa, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-238 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD222 molecules were detected at ~250 kDa in all analysed cell lines.

Quick Database Links

Gene Symbol

CD222

UniProt

P11717

NCBI Gene ID

3482

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation