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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 7
| Tested Applications | FC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | MEM-181 |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Target | CD25 |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−IL-2R α chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR
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Separation of CD3 positive CD25 positive lymphocytes (red-filled) from CD3 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using anti-human CD25 PE antibody (concentration in sample 0.5 µg/mL).
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD25 PE antibody (concentration in sample 0.5 µg/mL) and anti-human CD4 APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD25 PE antibody (concentration in sample 0.5 µg/mL) and anti-human CD3 APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Expression profiling on peripheral blood subsets using Anti-human CD25 PE antibody. Adaptive panel.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD25 PE antibody (concentration in sample 0.5 µg/mL).
Separation of CD4 positive CD25 positive Treg cells (red-filled) from CD4 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD25 PE antibody (concentration in sample 0.5 µg/mL).
Expression profiling on peripheral blood subsets using Anti-human CD25 PE antibody. Innate panel.
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CD25 Antibody (PE) (orb44158)
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