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CD271 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IHC-P, IP, WB |
|---|---|
| Reactivity | Feline, Ferret, Human, Primate, Rabbit |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | NGFR5 |
| Immunogen | Purified CD271 protein isolated from human melanoma cell line A875 |
| Target | CD271 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Mass cytometry (surface staining) of brain tumor cells with anti-CD271 (NGFR5) 143Nd. Gated on singlets.

Separation of SK-MEL-30 cells stained using anti-CD271 (NGFR5) purified antibody (concentration in sample 1.7 µg/ml, GAM APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Immunohistochemistry staining of human testis (paraffin sections) using anti-CD271 (NGFR5).
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Protocol Information
CD271 Antibody (orb154382)
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