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CD271 Antibody

SKU: orb154382

Description

Mouse monoclonal antibody to CD271 which is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells.

Research Area

Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsFC, ICC, IHC-P, IP, WB
ReactivityFeline, Ferret, Human, Primate, Rabbit
Application Notes
Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.NGFR5
ImmunogenPurified CD271 protein isolated from human melanoma cell line A875
TargetCD271
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TNFRSF16, NGFR, p75NTR, p75NGFR

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CD271 Antibody

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CD271 Antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

CD271 Antibody (orb154382)

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Available Sizes

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0.1 mg
$ 280.00
DispatchUsually dispatched within 5-10 working days weeks.
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