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CD45RB Antibody
SKU: orb44073
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-143 |
| Immunogen | Human peripheral blood lymphocytes. |
| Target | CD45RB |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−PTPRCR, T200RB
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Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45RB (MEM-143) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45RB (MEM-143) purified antibody (concentration in sample 0.6 µg/ml) GAM APC.
Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD45RB (clone MEM-143).
Anti-Hu CD45RB Purified (clone MEM-143) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Ramos, Molt-4, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-143 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD45RB molecules were detected at ~180-260 kDa in Jurkat and Ramos cell line under reducing and non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
CD45RB Antibody (orb44073)
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