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CD59 Antibody
SKU: orb44303
Description
Research Area
Epigenetics, Stem Cells
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, IP, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | MEM-43/5 |
| Immunogen | Thymocytes and T lymphocytes |
| Target | CD59 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN
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Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate; Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. orb44294)
Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 µg/ml, GAM APC).
Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IP
Immunoprecipitation
CD59 Antibody (orb44303)
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