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p53 (pS392) Antibody
SKU: orb43788
Description
Research Area
Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | FP3.2 [FPS392] |
| Immunogen | KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53. |
| Target | p53 (pS392) |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BCC7, TRP53, TP53, LFS1
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![Mouse Hu p53 (pS392) Purified, clone FP3.2 [FPS392] (Monoclonal) Antibody](/images/quality_badge_antibodies.png)
Mouse Hu p53 (pS392) Purified, clone FP3.2 [FPS392] (Monoclonal) Antibody [orb1882377]
IHC-P, WB
Human
Mouse
Monoclonal
Unconjugated
0.1 mg
Quality Guarantee
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Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). A - anti-p53 (total); B - anti-p53 (phospho Ser392). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.
Western blotting analysis of phosphorylated human p53 using mouse monoclonal antibody FP3.2 (purified) in T47D cell line, which expresses p53. A) Phosphorylated p53 detected in T47D cell line. B) Absence of phosphorylated signal of p53 in dephosphorylated T47 cells (pretreated with calf intestinal alkaline phosphatase).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
p53 (pS392) Antibody (orb43788)
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