Anti-HVEM [HMHV-1B18]

SKU: orb758794

Description

Rabbit monoclonal antibody to HVEM

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	Blocking, FA, FC, IP, WB
Reactivity	Mouse

Key Properties

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Host	Hamster
Clonality	Monoclonal
Isotype	Hamster IgG
Clone No.	HMHV-1B18
Immunogen	This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
Target	HVEM
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD270; TNF Receptor-like 2; ATAR; Herpes virus Entry Mediator A; HVEA; Herpes virus Entry Mediator; LIGHTR; Tumor Necrosis Factor Receptor-Like Gene2; Tumor Necrosis Factor Receptor-Like 2; TR2; Tumor Necrosis Factor Receptor Superfamily Member 14; TNFRSF14; CD40-Like Protein

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Flow cytometry using the Anti-HVEM antibody HVEM-1B18. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of HVEM-1B18 (orb758794, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-HVEM antibody HMHV-1B18. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (orb758794) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758794, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-HVEM antibody HMHV-1B18 Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (orb758794) at 0.01 µg/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

HVEM

UniProt

Q71F55

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation