IFN gamma Antibody

SKU: orb154407

Description

Mouse monoclonal antibody to IFN-gamma which belongs to the type II (or gamma) interferon family. IFN-gamma has antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells.Mutation in IFNG is associated with the risk of aplastic anemia (AA).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IP, RIA
Reactivity	Human
Application Notes	ELISA: Capture antibody in combination with detection antibody 4S.B3.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	NIB42
Immunogen	Recombinant human interferon gamma
Target	IFN gamma
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interferon gamma, IFN-gamma

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ELISA analysis of IFN gamma (7 different concentrations: 0.2 - 12.5 ng/ml) was performed using capture antibody NIB42 (1 μg/ml) and HRP-conjugated detection antibody 4S.B3 (1 μg/ml).

Quick Database Links

Gene Symbol

IFN gamma

UniProt

P01579

NCBI Gene ID

3458

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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RIA

Radioimmunoassay

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