Anti-Interleukin-6 (IL-6) Mouse

SKU: orb1125577

Description

Rabbit polyclonal antibody to Interleukin-6 (IL6)

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100Âµl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Reactivity	Mouse
Application Notes	Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-6 (0.1 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required. ELISA:To detect mIL-6 by direct ELISA (using 100Âµl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-6. Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	highly pure (>98%) recombinant mouse IL-6
Purity	Affinity purified
Purification	Affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS buffer, pH 7.4. Reconstitute in sterile water at a concentration of >0.2 mg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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