JNK1/2/3 (Phospho-T183/Y185) Rabbit Polyclonal Antibody

SKU: orb315601

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Description

The JNK1/2/3 (Phospho-T183/Y185) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	IF, WB
Dilution Range	WB: 1-500:1000
Reactivity	Gallus, Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
Target	MAPK8; MAPK9; MAPK10
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK10; JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3

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JNK1/2/3 (Phospho-T183/Y185) Rabbit Polyclonal Antibody

Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in BV2 (A), MCF7 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 46; 54 kD)

Immunofluorescent analysis of JNK1/2/3 (Phospho-T183/Y185) staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MAPK8; MAPK9; MAPK10

UniProt

P49187, P45984, Q91Y86, Q9WTU6, P49185, Q61831, P53779, P49186, P45983

NCBI Gene ID

5601, 26420, 25272, 26419, 50658, 5602, 116554, 5599

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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