L-Arginine decarboxylase/AZIN2 Rabbit Polyclonal Antibody

SKU: orb381020

Description

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E. coli-derived human AZIN2 recombinant protein (Position: C111-Q301). Human AZIN2 shares 89.4% amino acid (aa) sequence identity with mouse AZIN2.
Target	Antizyme inhibitor 2
Molecular Weight	39 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Antizyme inhibitor 2; AzI2; Arginine decarboxylase; ADC; ARGDC; Ornithine decarboxylase-like protein; ODC-like protein; ornithine decarboxylase paralog; ODC-p; AZIN2; ADC, KIAA1945, ODCP

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L-Arginine decarboxylase/AZIN2 Rabbit Polyclonal Antibody

AZIN2 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.

AZIN2 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.

IHC analysis of AZIN2 using anti-AZIN2 antibody. AZIN2 was detected in paraffin-embedded section of human testicar cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-AZIN2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of AZIN2 expression in rat brain extract (lane 1), mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). AZIN2 at 50KD was detected using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method.

Quick Database Links

Gene Symbol

Antizyme inhibitor 2

UniProt

Q96A70

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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