Ly-49C/F/H/I Antibody-PE labled

SKU: orb1293662

Description

The Ly-49C/F/H/I Antibody-PE labled is suitable for FC, IF. It is a Monoclonal, PE conjugated antibody which raised against IL-2-activated killer cells (LAK) from C57BL/6 mice. Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Golden Hamster
Clonality	Monoclonal
Clone No.	14B11
Immunogen	IL-2-activated killer cells (LAK) from C57BL/6 mice
Purification	The antibody was purified by affinity chromatography.
Conjugation	PE

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Syrian Hamster IgG. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Ly-49c; Ly49C; Killer cell lectin-like receptor 3; 5E6; Lymphocyte antigen 49c; Ly-49c; Nk2.1; T-cell surface glycoprotein Ly-49C; Ly-49f; Ly49-f; Ly49F; Killer cell lectin-like receptor 6; Lymphocyte antigen 49f; Ly-49f; T-cell surface glycoprotein Ly-49F; Ly-49h; Ly49-h; Ly49H; Killer cell lectin-like receptor 8; Lymphocyte antigen 49h; Ly-49h; T-cell surface glycoprotein Ly-49H; Killer cell lectin-like receptor subfamily A member 9; Killer cell lectin-like receptor subfamily A member 9; Natural killer cell recptor Ly49C

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Quick Database Links

UniProt

Q60682, Q2TJJ8, Q64329, Q60653

NCBI Gene ID

16640, 16637, 16634, 16639

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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