MMP12 Rabbit Monoclonal Antibody

SKU: orb547774

Description

Anti-MMP12 Rabbit Monoclonal Antibody. Tested in WB application. This antibody reacts with Human.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB 1:500-2000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	CHE-13
Immunogen	A synthesized peptide derived from human MMP12
Target	Macrophage metalloelastase
Molecular Weight	43 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME

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Western blot analysis of MMP12 expression in WI-38 cell lysate (orb547774). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP12 monoclonal antibody (Catalog # orb547774) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for MMP12.

Quick Database Links

Gene Symbol

Macrophage metalloelastase

UniProt

P39900

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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