Anti-Mouse antibody kappa light chain [OX-20]

ELISA using OX-20, orb256381, orb256388 and orb256398. Binding curves of the rabbit IgG chimeric version of the Anti-HA antibody OX-20 against orb256381 (blue line), orb256388 (orange line) and orb256398 (grey line) to an ELISA plate coated with orb256381, orb256388 and orb256398 at a concentration of 2.5 ug/ml.

Flow cytometry using the Anti-Mouse antibody kappa light chain antibody OX-20. mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of OX-20 (orb2645677, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed mouse splenocytes with anti-Mouse antibody kappa light chain antibody OX-20. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of OX-20 (orb2645677) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645677, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Mouse antibody kappa light chain antibody OX-20 Mouse spleen (A) and mouse thymus (B) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OX-20 (orb2645677) at 0.001 µg/ml and 0.0001 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.