CD16/CD32 Antibody (FITC)

SKU: orb623582

Description

Rat monoclonal antibody against CD16 conjugated to FITC

Images & Validation

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Item 1 of 2

Tested Applications	FC
Reactivity	Mouse
Application Notes	Flow cytometry: Recommended dilution: 2-5 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Rat IgG2a lambda
Clone No.	93
Immunogen	Murine pre-B cells
Target	CD16/CD32
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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CD16, CD32, FcgammaRIII, FCgammaRII, FCGR3, FCGR2

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Separation of murine CD16/32 positive cells (red-filled) from murine CD16/32 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 µg/ml).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 µg/ml).

Quick Database Links

Gene Symbol

CD16/CD32

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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