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Myc-tag Antibody
SKU: orb323058
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:2000-1:5000, IF/ICC: 1:200-1:500, IP: 1:100-1:200 |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of Myc-tag. The exact sequence is proprietary. |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate. (Predicted band size: \; Observed band size: \)
Immunofluorescent analysis of Myc-tag staining in 293T cells transfected with a Myc-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation of Myc-tagged protein from HEK293T cells transfected with vector overexpressing Myc tag, using Anti-Myc-tag Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
Myc-tag Antibody (orb323058)
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