Nck NCK1 Rabbit Monoclonal Antibody

SKU: orb547988

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IF, IHC, IP, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:50 FC 1:500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	AFEG-14
Immunogen	A synthesized peptide derived from human Nck Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Target	SH2/SH3 adapter protein NCK1
Molecular Weight	16 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Oligodendrocyte transcription factor 2; Oligo2; Class B basic helix-loop-helix protein 1; bHLHb1; Class E basic helix-loop-helix protein 19; bHLHe19; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; OLIG2; BHLHB1, BHLHE19, PRKCBP2, RACK17

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Western blot analysis of Nck expression in HeLa cell lysate.

Quick Database Links

Gene Symbol

SH2/SH3 adapter protein NCK1

UniProt

P16333

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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