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NECTIN4 Biosimilar Antibody
SKU: orb1291227
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Description
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−Item 1 of 3
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1:5000-10000 Flow Cyt 1:100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target | NECTIN4 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Disclaimer | For research use only |
Alternative Names
−EDSS1LNIRnectin-4PRR4PVRL4
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ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag orb1173741 can bind Anti-Nectin-4 Neutralizing antibody (orb1291227) in a linear range of 0.61–39.06 ng/ml.
Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (orb1291227) on Expi293 cells transfected with Human Nectin-4 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb (orb1291227). (A) orb1291227 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of orb1291227 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of orb1291227 to Nectin-4. Antibodies were incubated at 2 μg/mL.
Quick Database Links
Gene Symbol
NECTIN4
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
NECTIN4 Biosimilar Antibody (orb1291227)
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