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Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IHC-P, WB |
|---|---|
| Reactivity | Mammal |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | NF-01 |
| Immunogen | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| Target | Neurofilament H |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NFH, NEFH, Neurofilament heavy chain, CMT2CC
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Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-neurofilament heavy protein (NF-01).
Western blotting analysis of human neurofilament H protein using mouse monoclonal antibody NF-01 on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ICC
Immunocytochemistry
Neurofilament H Antibody (orb44465)
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