Neurofilament M Antibody

SKU: orb44467

Description

Mouse Monoclonal to Neurofilament medium protein.

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IHC-P, WB
Reactivity	Mammal
Application Notes	Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min. Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG2a
Clone No.	NF-09
Immunogen	Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Target	Neurofilament M
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NFM, NEFM, Neurofilament medium chain, NEF3

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Immunocytochemistry staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

Western blotting analysis of human neurofilament M protein using mouse monoclonal antibody NF-09 on lysates of HEK-293 cell line, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament M monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament M was detected around 160 kDa.

Quick Database Links

Gene Symbol

Neurofilament M

UniProt

P07197

NCBI Gene ID

4741

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ICC

Immunocytochemistry

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