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NHERF1 Antibody
SKU: orb43765
Description
Research Area
Epigenetics, Neuroscience, Signaling Pathways, Wnt
Images & Validation
−Item 1 of 1
| Tested Applications | IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | EBP-10 |
| Immunogen | Bacterially produced recombinant full-length human NHERF1. |
| Target | NHERF1 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−EBP50, NPHLOP2, SLC9A3R1, SR1
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Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IP
Immunoprecipitation
NHERF1 Antibody (orb43765)
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