Notch1 Rabbit Monoclonal Antibody

SKU: orb546994

Description

Anti-Notch1 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, IF, IHC, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 FC 1:50
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	BGA-14
Immunogen	A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.
Target	Neurogenic locus notch homolog protein 1
Molecular Weight	110 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

WB analysis of NOTCH1 expression in (1) HEK293 Cell; (2) K562 Cell; (3) U20S Cell; (4) Jurkat Cell; (5) HeLa Cell; (6) THP-1 Cell; (7) CACO-2 Cell; (8) rat brain tissue; (9) PC-12 Cell; (10) mouse lung tissue; (11) mouse brain tissue tissue.

Immunofluorescent analysis of HeLa cells, using Notch1 Antibody.

Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody.

Quick Database Links

Gene Symbol

Neurogenic locus notch homolog protein 1

UniProt

P46531

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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