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Occludin/OCLN Rabbit Polyclonal Antibody
SKU: orb234284
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Western blot, 0.1-0.5μg/ml, Human |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin. |
| Target | Occludin |
| Molecular Weight | 59 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Occludin; OCLN
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Anti-Occludin antibody, Western blotting. All lanes: Anti Occludin at 0.5 ug/ml. Lane 1: SW620 Whole Cell Lysate at 40 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug Lane 5: U87 Whole Cell Lysate at 40 ug. Predicted bind size: 59 KD. Observed bind size: 59 KD.
Anti-Occludin antibody, IHC(P): Human Mammary Cancer Tissue.
Quick Database Links
Gene Symbol
Occludin
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Occludin/OCLN Rabbit Polyclonal Antibody (orb234284)
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