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Anti-PD-L1 [332M7]

SKU: orb1152460

Description

Rabbit monoclonal antibody to PD-L1

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsBlocking, FC
ReactivityHuman, Monkey, Mouse

Key Properties

HostHuman
ClonalityMonoclonal
IsotypeHuman IgG
Clone No.332M7
ImmunogenThe original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions.
TargetPD-L1
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1

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Anti-PD-L1 [332M7]

Flow cytometry using the Anti-PD-L1 antibody 332M7. Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 332M7 (orb1152460, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Anti-PD-L1 [332M7]

Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 332M7. Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (orb1152460) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152460, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

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Protocol Information

FC
Flow Cytometry
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Anti-PD-L1 [332M7] (orb1152460)

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Available Sizes

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0.2 mg
$ 600.00
DispatchUsually dispatched within 5-10 working days
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