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Anti-PD-L1 [332M7]
SKU: orb1152461
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, FC |
|---|---|
| Reactivity | Human, Monkey, Mouse |
Key Properties
−| Host | Human |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 332M7 |
| Immunogen | The original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions. |
| Target | PD-L1 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1
Similar Products
−- Item 1 of 2
![Anti-PD-L1 [332M7]](/images/pub/media/catalog/product/NewWebsite/35/orb1152460_1.png)
![Anti-PD-L1 [332M7]](/images/pub/media/catalog/product/NewWebsite/35/orb1152460_2.png)
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![Anti-PD-L1 [332M7]](/images/pub/media/catalog/product/NewWebsite/35/orb1152461_1.png)
Flow cytometry using the Anti-PD-L1 antibody 332M7. Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 332M7 (orb1152460, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-PD-L1 [332M7]](/images/pub/media/catalog/product/NewWebsite/35/orb1152461_2.png)
Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 332M7. Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (orb1152460) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152460, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
PD-L1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Anti-PD-L1 [332M7] (orb1152461)
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