Phospho-DNA PKcs PRKDC/DNA Rabbit Monoclonal Antibody

SKU: orb548611

Description

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	BOD-16
Immunogen	A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
Target	DNA-dependent protein kinase catalytic subunit
Molecular Weight	185 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; 2.7.11.1; DNPK1; p460; PRKDC; HYRC, HYRC1

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Phospho-DNA PKcs PRKDC/DNA Rabbit Monoclonal Antibody

Western blot analysis of Phospho-DNA PKcs (Ser2056) expression in alkaline treated Jurkat cell lysate.

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-DNA PKcs (S2056) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Phospho-DNA PKcs (S2056) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.