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Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal Antibody
SKU: orb548623
Description
Research Area
Epigenetics & Chromatin, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IP, WB |
|---|---|
| Dilution range | WB 1:500-2000 ICC/IF 1:50-200 IP 1:20 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | EDE-18 |
| Immunogen | A synthesized peptide derived from human Phospho-RSK1 (S380) |
| Target | Ribosomal protein S6 kinase alpha-1 |
| Molecular Weight | 80 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin. |
| Concentration | 0.5mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 2.7.11.1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1; MAPKAPK1A, RSK1
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Western blot analysis of Phospho-RSK1 (S380) expression in A431 cell lysate treated with EGF (orb548623). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA1 monoclonal antibody (Catalog # orb548623) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for RPS6KA1.
Quick Database Links
Gene Symbol
Ribosomal protein S6 kinase alpha-1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
Phospho-RSK1 (S380) RPS6KA1 Rabbit Monoclonal Antibody (orb548623)
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