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Phospho-Smad3 Rabbit Monoclonal Antibody

SKU: orb548575

Description

Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsICC, IF, IHC, WB
Dilution RangeWB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
ReactivityHuman, Mouse

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone No.AHO-19
ImmunogenA synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
TargetMothers against decapentaplegic homolog 3
Molecular Weight90-100 kDa
PurificationAffinity-chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesRabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration0.5mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Phospho-Smad3 Rabbit Monoclonal Antibody

Immunofluorescent analysis of A549 cells treated with TGFβ, using Phospho-Smad3 (S423 + S425) Antibody.

Phospho-Smad3 Rabbit Monoclonal Antibody
Phospho-Smad3 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

Phospho-Smad3 Rabbit Monoclonal Antibody (orb548575)

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Available Sizes

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100 μl
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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