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Phospho-Smad3 Rabbit Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | AHO-19 |
| Immunogen | A synthesized peptide derived from human Phospho-Smad3 (S423 + S425) |
| Target | Mothers against decapentaplegic homolog 3 |
| Molecular Weight | 90-100 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Phospho-Smad3 (Ser423 + Ser425) Recombinant Rabbit Monoclonal Antibody [orb526653]
WB
Porcine, Rat
Human, Mouse
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl

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Immunofluorescent analysis of A549 cells treated with TGFβ, using Phospho-Smad3 (S423 + S425) Antibody.


Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using Phospho-Smad3 (S423 + S425) Antibody.
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Protocol Information
Phospho-Smad3 Rabbit Monoclonal Antibody (orb548575)
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