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PPM1D Antibody
SKU: orb1467381
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, ICC, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7E11/C5 |
| Immunogen | Purified human PPM1D |
| Target | PPM1D |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−WIP1, Protein phosphatase 1D, JDVS, WIP1, IDDGIP
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Western blotting analysis of human PPM1D using mouse monoclonal antibody 7E11/C5 on lysates of U2OS parental cells, expressing both natural (70 kDa, low expression) and C-terminally truncated version (55 kDa, high expression) of PPM1D, and on lysates of MCF7 cells (high PPM1D expression) and RPE cells (low PPM1D expression); reducing conditions.
Separation of MCF-7 cells stained using anti-PPM1D (7E11/C5) purified antibody (concentration in sample 2 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PPM1D Antibody (orb1467381)
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