Anti-PRMT3 [RAB-C372]

SKU: orb1089926

Description

Human polyclonal antibody to PRMT3

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	ChIP, ELISA
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	Fab fragment
Clone No.	RAB-C372
Immunogen	This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT3 protein under non-denaturing conditions.
Target	PRMT3
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; Protein arginine N-methyltransferase 3; anti-PRMT3-RAB-C372; 38D1_11B

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Immunofluorescence staining of fixed MCF7 cells with anti-PRMT3 antibody RAB-C372. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-C372 (orb1089949) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089949, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-PRMT3 antibody RAB-C372 HEK293(A), HeLa(B), HepG2(C), MCF7(D) and A549(E) cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C372 (orb1089949) at 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

PRMT3

UniProt

O60678

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ChIP

Chromatin Immunoprecipitation

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