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RAP80 Rabbit Monoclonal Antibody
SKU: orb548411
Description
Research Area
Epigenetics & Chromatin, Molecular Biology, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | ADCH-21 |
| Immunogen | A synthesized peptide derived from human RAP80 Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs) . |
| Target | BRCA1-A complex subunit RAP80 |
| Molecular Weight | 70,90 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin. |
| Concentration | 0.5mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD266; TNFRSF12A; FN14
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Western blot analysis of UIMC1 expression in 293T cell lysate using anti-UIMC1 antibody (orb548411). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UIMC1 antigen affinity purified polyclonal antibody (Catalog # orb548411) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for UIMC1.
Quick Database Links
Gene Symbol
BRCA1-A complex subunit RAP80
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
RAP80 Rabbit Monoclonal Antibody (orb548411)
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