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CD28 Antibody (FITC)

SKU: orb154479

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD28 that is expressed constitutively on virtually 100% of mouse Tcells and on developing thymocytes. . Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs.

Images & Validation

Tested ApplicationsFC
ReactivityRat
Application Notes
Flow cytometry: Recommended dilution: 2 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.JJ319
ImmunogenMouse A20J B lymphoma cells transfected with rat CD28
TargetCD28
PurificationPurified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationFITC

Storage & Handling

StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

Tp44

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CD28 Antibody (FITC)

Flow cytometry analysis (surface staining) of rat thymocytes (red-filled) with anti-rat CD28 (JJ319) FITC and unstained rat thymocytes (grey-filled).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

CD28 Antibody (FITC) (orb154479)

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0.1 mg
$ 330.00
DispatchUsually dispatched within 5-10 working days weeks.
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