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CD28 Antibody (PE)

SKU: orb154508

Description

Mouse monoclonal antibody conjugated to PE which recognizes CD28 that is expressed constitutively on virtually 100% of mouse Tcells and on developing thymocytes. . Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs.

Images & Validation

Tested ApplicationsFC
ReactivityRat
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.JJ319
ImmunogenMouse A20J B lymphoma cells transfected with rat CD28
TargetCD28
PurificationPurified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationPE

Storage & Handling

StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

Tp44

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CD28 Antibody (PE)

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD28 (JJ319) PE antibody (concentration in sample 0.56 μg/ml).

CD28 Antibody (PE)

Flow cytometry surface staining pattern of rat splenocytes stained using anti-rat CD28 (JJ319) PE antibody (concentration in sample 0.56 μg/ml).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

CD28 Antibody (PE) (orb154508)

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0.1 mg
$ 360.00
DispatchUsually dispatched within 5-10 working days weeks.
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