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CD8b Antibody (PE)

SKU: orb154509

Description

Mouse monoclonal antibody conjugated to PE which recognizes CD8B that also known as P37 and LEU2. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

Research Area

Epigenetics, Immunology

Images & Validation

Tested ApplicationsFC
ReactivityRat
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.341
ImmunogenCD8 positive Wistar rat splenic T cell hybridomas
TargetCD8b
PurificationPurified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationPE

Storage & Handling

StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Ly-3, Ly-C, Lyt-3

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CD8b Antibody (PE)

Separation of rat CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) rat splenocyte suspension stained using anti-rat CD8b (341) PE antibody (concentration in sample 3 μg/ml).

CD8b Antibody (PE)

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) PE antibody (concentration in sample 3 μg/ml).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

CD8b Antibody (PE) (orb154509)

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Available Sizes

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0.1 mg
$ 360.00
DispatchUsually dispatched within 5-10 working days weeks.
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