SEMA4D/CD100 Antibody

SKU: orb1806110

Description

Anti-SEMA4D / CD100 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Clonality	Monoclonal
Target	SEMA4D / CD100
Source/Expression System	CHO
Molecular Weight	145.88 kDa
Purity	>95%
Purification	Protein A
Endotoxin	<0.001 EU/ug
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100 mM Proline/Acetic acid 20mM Arginine pH 5.0
Concentration	Batch dependent
Disclaimer	For research use only

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Anti-SEMA4D / CD100 Reference Antibody (pepinemab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-SEMA4D / CD100 Reference Antibody (pepinemab)is more than 100% ;determined by SEC-HPLC.

Immobilized human SEMA4D/CD100; His Tag at 2 μg/mL can bind Anti-SEMA4D / CD100 Reference Antibody (pepinemab);EC50=0.0081μg/mL.

Human C100 HEK293 cells were stained with Anti-SEMA4D / CD100 Reference Antibody (pepinemab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC231=0.3227 nM.

Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects; with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.

Quick Database Links

Gene Symbol

SEMA4D / CD100

UniProt

Q92854

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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