Skp1 Rabbit Monoclonal Antibody

SKU: orb547336

Description

Research Area

Cell Biology, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IF, WB
Dilution Range	WB 1:500-1:2000 ICC/IF 1:50-1:200 FC 1:100
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	ADDF-19
Immunogen	A synthesized peptide derived from human Skp1 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
Target	S-phase kinase-associated protein 1
Molecular Weight	501 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DGCR8; C22orf12, DGCRK6; LP4941

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WB analysis of SKP1 expression in (1) 293T Cell; (2) NIH/3T3 Cell using anti-SKP1 antibody.

Quick Database Links

Gene Symbol

S-phase kinase-associated protein 1

UniProt

P63208

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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