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TM4SF1 Biosimilar Antibody
SKU: orb1743394
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC |
|---|---|
| Dilution range | Flow Cyt 1:100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human Humanized |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target | TM4SF1 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−M3S1TAAL6
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Flow cytometry analysis with 1 μg/mL Anti-TM4SF1 (AGX101 biosimilar) mAb (orb1743394) on Expi293 cells transfected with Human TM4SF1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plates were pre-coated with 0.5 μg/per well purified human TM4SF1 full length membrane nanoparticles (Cat# orb1743250). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb binding with TM4SF1 MNPs (Cat# orb1743250) is 4.174 ng/ml.
ELISA plates were pre-coated with Flag Tag TM4SF1-Nanodisc (Cat# orb1743247, 0.2 μg/per well). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) binding with TM4SF1-Nanodisc (Cat# orb1743247) is 15.97 ng/ml.
FACS analysis of TM4SF1 MNPs (Cat# orb1743250).A. TM4SF1 MNP (Cat# orb1743250) were stained only with Goat anti-human 2nd antibody.B. Negative control MNP samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody.C. TM4SF1 MNP (Cat# orb1743250) samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2 μg/mL, followed by Goat anti-human 2nd antibody.D. TM4SF1 MNP (Cat# orb1743250) samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) at 2 μg/mL, followed by Goat anti-human 2nd antibody.
Quick Database Links
Gene Symbol
TM4SF1
UniProt
UniProt Details
− No UniProt data available
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TM4SF1 Biosimilar Antibody (orb1743394)
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