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TNF alpha Antibody (Biotin)

SKU: orb1480525

Description

Mouse monoclonal antibody against TNF conjugated to Biotin

Images & Validation

Tested ApplicationsELISA, FC, ICC, IHC-Fr
ReactivityHuman, Porcine, Primate
Application Notes
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow cytometry: Intracellular staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.MAb11
ImmunogenRecombinant human TNF-alpha
TargetTNF alpha
PurificationPurified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
ConjugationBiotin

Storage & Handling

StorageStore at 2-8°C. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

TNFA, DIF, TNLG1F

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TNF alpha Antibody (Biotin)

ELISA analysis of TNF alpha (0 - 4.1 ng/ml) using capture antibody MAb1 (5 μg/ml) and biotinylated detection antibody MAb11 (0.15 - 10 μg/ml).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
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ICC
Immunocytochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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TNF alpha Antibody (Biotin) (orb1480525)

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0.1 mg
$ 360.00
DispatchUsually dispatched within 5-10 working days
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