TNF alpha Antibody (FITC)

SKU: orb179929

Description

Mouse monoclonal antibody against TNF alpha conjugated to FITC.

Research Area

Cancer, Epigenetics, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 3

Tested Applications	FC
Reactivity	Human, Porcine, Primate
Application Notes	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	MAb11
Immunogen	Recombinant human TNF-alpha
Target	TNF alpha
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Disclaimer	For research use only

Alternative Names

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TNFA, DIF, TNLG1F

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Flow cytometry multicolor intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension). For surface staining was used anti-human CD3 (UCHT1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human TNF alpha (MAb11) FITC antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

TNF alpha

UniProt

P01375

NCBI Gene ID

7124

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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