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TNFRSF10B Biosimilar Antibody
SKU: orb757417
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1:5000-10000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human Humanized |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target | TNFRSF10B |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Disclaimer | For research use only |
Alternative Names
−TNFRSF10BTRAILR2TRAIL-R2CD262DR5KILLERTRICK2ZTNFR9TRICKB
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human TNFRSF10B, mFc tagged protein orb689451 can bind Anti-TNFRSF10B Neutralizing antibody in a linear range of 3.2-80 ng/ml.
TNFRSF10B protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-TNFRSF10B (tigatuzumab) on Expi293 cells transfected with human TNFRSF10B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)
Flow cytometry analysis of antigen binding of anti-human TNFRSF10B mAb (orb757417). (A) orb757417 does not bind to TT cells that do not express TNFRSF10B. (B) A clear peak shift of orb757417 was seen compared to the control when incubated with TNFRSF10B-expressing SNU-5 cells, indicating strong binding of orb757417 to TNFRSF10B. Antibodies were incubated at 5 μg/mL.
Quick Database Links
Gene Symbol
TNFRSF10B
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNFRSF10B Biosimilar Antibody (orb757417)
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