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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB: 0.736111111 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 of mouse troponin 1, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH). |
| Target | Troponin I (cardiac) Ser23/24 |
| Molecular Weight | 25 |
| Purification | Antigen Affinity Purified from Pooled Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol. |
| Concentration | Lot Dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Western blots showing specific labeling of S23/S24 TnI, cat. orb319692 (upper) and TnI; Cat. orb319691 (lower) in protein lysates of mouse cardiomyocytes.
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UniProt Details
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Protocol Information
Troponin I (cardiac) Ser23/24 Antibody (orb319692)
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