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TSG6/TNFAIP6 Rabbit Polyclonal Antibody

SKU: orb389513

Description

Anti-TSG6/TNFAIP6 Antibody. Tested in WB applications. This antibody reacts with Human, Rat.

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

Tested ApplicationsWB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human, Rat
ReactivityHuman, Rat

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminus of human TSG6, different from the related mouse sequence by two amino acids.
TargetTumor necrosis factor-inducible gene 6 protein
Molecular Weight31 kDa.
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Tumor necrosis factor-inducible gene 6 protein; Hyaluronate-binding protein; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6; TNF alpha-induced protein 6; TNFAIP6; TSG6

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TSG6/TNFAIP6 Rabbit Polyclonal Antibody

Western blot analysis of TSG6 using anti-TSG6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSG6 at approximately 31 kDa. A specific band was detected for TSG6 at approximately 31 kDa.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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TSG6/TNFAIP6 Rabbit Polyclonal Antibody (orb389513)

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100 μg
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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