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UBE2F Rabbit Polyclonal Antibody
SKU: orb2804165
Description
Images & Validation
−
| Tested Applications | ELISA, FC, ICC, IHC, WB |
|---|---|
| Dilution Range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human UBE2F recombinant protein (Position: M1-R185). Human UBE2F shares 96.8% and 96.2% amino acid (aa) sequence identity with mouse and rat UBE2F, respectively. |
| Target | NEDD8-conjugating enzyme UBE2F |
| Molecular Weight | 21 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NCE2; EC:2.3.2.32; NEDD8-conjugating enzyme 2; NEDD8 protein ligase UBE2F; NEDD8 conjugating enzyme 2
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Quick Database Links
Gene Symbol
NEDD8-conjugating enzyme UBE2F
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
UBE2F Rabbit Polyclonal Antibody (orb2804165)
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