Anti-VEGF [A.4.6.1]

SKU: orb348842

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Description

Mouse monoclonal antibody to VEGF

Research Area

Cancer Biology

Images & Validation

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Item 1 of 3

Tested Applications	Blocking, ELISA, FC, IHC, IP, WB
Reactivity	Human, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	A.4.6.1
Immunogen	Recombinant human VEGF.
Target	VEGF
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VEGF-A; Vascular Endothelial Growth Factor; VEGFA; Vascular Permeability Factor; VPF

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Immunohistochemical staining of rat brain tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat brain tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 (orb348842) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat skin tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat skin tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 (orb348842) was used to stain samples at a concentration of 5 µg/ml.

Western Blot using anti-VEGF antibody. Rat skin lysate was resolved on a 10% SDS PAGE gel and blots probed with orb348842 at 1.0 µg/ml before being detected by a secondary antibody. The expected band size (27 kDa) is seen along with bands corresponding to dimers.