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VEGFR1 Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide of human VEGF Receptor 1 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin. |
| Disclaimer | For research use only |
Alternative Names
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−VEGFR1 Rabbit Polyclonal Antibody [orb11556]
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50 μl, 100 μl, 200 μlFlt-1 (phospho Tyr1333) rabbit pAb Antibody [orb764360]
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100 μl, 50 μlRat Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit [orb779853]
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0.16-10 ng/mL
0.059 ng/mL
96 T, 48 TPig Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit [orb781761]
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0.32-20 ng/mL
0.119 ng/mL
48 T, 96 TMouse Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit [orb774943]
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0.16-10 ng/mL
0.059 ng/mL
96 T, 48 T

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Western blot analysis of VEGFR1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 151 kD; Observed band size: 180 kD)

Immunohistochemical analysis of VEGFR1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.63). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
VEGFR1 Antibody (orb1474481)
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