Apoptosis Activator 2

SKU: orb1223995

Description

An apoptosis activator that strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation; leads to the destruction of cells (Apaf-1 dependent) with IC50 of 4 uM; exert cytostatic and cytotoxic effects on a variety of cancer cell lines while having little or no activity against the normal cell lines tested.

Images & Validation

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Key Properties

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CAS Number	79183-19-0
MW	306.1435
Purity	>98% (HPLC)
Formula	C₁₅H₉Cl₂NO₂
SMILES	O=C1N(CC2=CC=C(Cl)C(Cl)=C2)C3=C(C=CC=C3)C1=O
Target	Apoptosis
Solubility	DMSO: ≥ 46 mg/mL

Bioactivity

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In Vitro

MDK83190 (Compound 2) (1-50 μM; 2 hours) effects against cell lines of lymphoid origin (CCRF-CEM, MOLT-4, and Jurkat) with IC50 values ranging from 4 to 9 μM. MDK83190 (Compound 2) (20 μM; 30 mins) activates procaspase-9 and procaspase-3 in a cyto c-dependent manner. MDK83190 (Compound 2) (0.1-100 μM; 22 hours)strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation, and finally killing cells with an IC50 of 4 μM. Cell Viability Assay Cell line: Leukemia cell line: CCRF-CEM cells, MOLT-4 cells, and Jurkat cells. Concentration: 1-50 μM. Incubation time: 2 hours. Result: Was sensitive to compound 2-induced apoptosis. Western blot analysis. Cell line: Hela cells. Concentration: 20 μM. Incubation time: 30 mins. Result: Induced procaspase-9 and procaspase-3 to active forms. Apoptosis Analysis Cell line: Jurkat cells. Concentration: 0.1-100 μM Incubation time: 22 hours. Result: Resulted inapoptosis and finally killed cells.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Gene Symbol

Apoptosis

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