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Featured
KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IHC-P, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Guinea pig, Porcine, Rabbit, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases.The immunogen is located within amino acids 650 - 700 of APP. |
| Target | APP |
| Molecular Weight | Predicted: 83 kDa Observed: 115 kDa |
| Purification | APP Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | APP Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
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APP KO Validation in 293T Cells, Loading: 10 µg of lysate Antibodies: APP orb1239089, 0.5 µg/mL and beta-actin, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western blot analysis of APP in (A) human, (B) mouse, and (C) rat brain tissue lysates with APP antibody at 1 µg/mL.
Immunohistochemistry of APP in human brain (Alzheimer's disease) tissue with APP antibody at 10 µg/mL.
Immunohistochemistry of APP in human brain tissue with APP antibody at 2.5 µg/mL.
Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 µg/mL. Green: APP Antibody (orb1239089), Blue: DAPI staining.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
APP Antibody (orb1239089)
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