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ARK-2 (phospho Thr232) rabbit pAb Antibody
SKU: orb770866
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AurB around the phosphorylation site of Thr232. AA range:198-247 |
| Molecular Weight | 39 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-AURKB antibody, anti-AIK2 antibody, anti-AIM1 antibody, anti-AIRK2 antibody, anti-ARK2 antibody, anti-STK1 antibody, anti-STK12 antibody, anti-STK5 antibody, anti-Aurora kinase B antibody, anti-Aurora 1 antibody, anti-Aurora- and IPL1-like midbody-associated protein 1 antibody, anti-AIM-1 antibody, anti-Aurora/IPL1-related kinase 2 antibody, anti-ARK-2 antibody, anti-Aurora-related kinase 2 antibody, anti-S antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AurB (Phospho-Thr232) Antibody.
Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7 cells treated with Nocodazole 1ug/ml 16h, using AurB (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ARK-2 (phospho Thr232) rabbit pAb Antibody (orb770866)
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