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Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated
SKU: orb348065
Description
Images & Validation
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| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:40,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Hamster |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Armenian Hamster IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum. Diminished reactivity will occur with Golden Syrian Hamster IgG. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Armenian Hamster IgG Antibody alkaline phosphatase Conjugation, goat anti-Armenian Hamster IgG alkaline phosphatase Conjugated Antibody, goat Anti-Hamster IgG alk phos Conjugated Secondary Antibody
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ELISA Results of Goat Anti-Armenian Hamster IgG Antibody Alkaline Phosphatase Conjugate tested against purified Armenian Hamster IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG (p/n orb346446). The working dilution is 1:4000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated (orb348065)
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